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Bite by Bite

Unraveling the Snakebite Crisis

Understanding the scale of the challenge is the first step toward the solution.

Deaths by Snakebite Envenoming

200,000
Annual Fatalities
43%
Indian Context
57%
Global Data

* Hover segments for detailed breakdown

Disproportionate Burden

India contributes to 43% of all global deaths caused by snakebite envenoming.

Lifetime Risk

In many Indian rural clusters, the lifetime risk of dying from a snakebite reaches an alarming 1 in 100.

System Inefficiencies

ASV Wastage
Shortage of ASV
Unindicated usage of ASV
Treatment Delay
Usage in non-envenomed patients

The complexity and unavailability of accurate detection methods make it difficult to treat snakebite victims promptly and remotely.

Identifying species is the critical first step
What is the Solution?

Correct and timely detection for Mixed Venom.

The Snake Venom Rapid Detection Test is specifically designed for Hemotoxic & Neurotoxic mixed detection, ensuring immediate life-saving intervention.

Innovative

Mixed Detection

Identifies both Hemotoxic and Neurotoxic venom types in a single rapid test.

Precise

High Sensitivity

Specific for Indian medical context with a detection limit of 10 ng/mL.

Rural First

Point of Care

Correct and timely detection at the bedside, optimized for rural clinics.

Reliable

Clinical Verification

Successfully verified across multiple healthcare settings and medical centers in India.

Snake Venom

Rapid Detection Kit
50 TESTS / BOX
100% SENSITIVITY
MIXED DETECTION
HEMO & NEURO
The Mechanism

How does it work?

The membrane technology leverages specialized capture zones to identify venom in the pre-symptomatic phase.

Step 01

Immersion & Complex

Sample interacts with first zone, forming a complex with ASV antibodies and gold particles.

Mobile Zone Interaction
Step 02

Capillary Action

The complex moves along the membrane strip within minutes of test application.

Test Zone Interaction
Step 03

Detection Capture

If venom is present, the complex is trapped here, producing a colored line in the 2nd zone.

Test Zone Interaction
Step 04

Control Confirmation

Unbound gold particles reach the control line (3rd zone) to confirm visibility.

Control Zone Interaction

The membrane has 3 distinct zones

First Zone

Mobile Zone

Colored colloidal gold particles sensitized with ASV Equine Polyclonal antibody.

Second Zone

Immobile Zone (Test Line)

Immobilized ASV Equine Polyclonal antibody that traps the venom-ASV complex.

Third Zone

Immobile Zone (Control Line)

Immobilized anti-mouse IgG which confirms the test functionality.

T
C
SNAKE
VENOM

Hover steps to see the specific membrane zone interaction. An absorbent pad is used to add the test sample for optimal movement through zones.

Step-by-Step

Clinical Testing Procedure.

Follow these simple clinical protocols to ensure 100% accurate results at the bedside. Designed for ease-of-use in emergency ward environments.

Time Critical Phase

Official readout window is between 2 to 20 minutes.

Testing Sequence

01

Collect the sample in a clean, dry container.

02

Add 1 drop (50 μl) of serum/plasma/whole blood into the sample window.

03

Add 1 drop of Test Diluent and allow to soak in.

04

Read results as early as 2 minutes but not beyond 20 minutes.

05

Do not interpret results beyond 20 minutes. Discard safely after use.

Safety Protocol

Discard the test device following biological safety and disposal protocols after single use.

Pre-Analytical Phase

Specimen Collection & Preparation

The kit supports multiple blood specimens, allowing for maximum flexibility in high-pressure emergency setup contexts.

Biological Safety

Avoid repeated freezing & thawing cycles.

Proper sample preparation is critical for maintaining 100% test specificity and sensitivity.

Serum (Yellow Top)

Clinical Protocol

Preparation Steps

Draw into clean, dry vial
Allow to clot fully
Centrifuge at 5000 rpm (15 mins)
Storage Protocol
2-8°C (For >3 days, freeze at -20°C)

Plasma (Purple Top)

Clinical Protocol

Preparation Steps

Collect using anticoagulants
Heparin, EDTA or Sodium Citrate
Centrifuge to obtain plasma
Storage Protocol
2-8°C (Avoid repetitive thawing)

Whole Blood (Immediate)

Clinical Protocol

Preparation Steps

Draw into vial with EDTA
Immediate specimen application
Allow 5 seconds to soak well
Storage Protocol
Store at 2-8°C (Use within 3 days)
Interpretation Guide

How to read the results.

The kit is designed for simple, immediate visual confirmation at the bedside. Follow the guide below to interpret the test window.

Negative Result

Snake Venom NOT Detected
T
C
SNAKE
VENOM

If only one line appears at the control (C) line, the test is negative.

Positive Result

Snake Venom Detected
T
C
SNAKE
VENOM

If two lines appear—one at the control (C) line and one at the test (T) line, the test is positive.

Invalid Result

Test Failed / Error
T
C
SNAKE
VENOM

If no lines appear, or if only the test (T) line appears without the control (C) line, the result is invalid.

Procedure Notice

Results should be read between 2 to 20 minutes.

Do not interpret results beyond the 20-minute window. If an invalid result occurs (no control line), re-test with a new device using a freshly collected sample.

Product Deep Dive

Built for Extreme Precision.
Optimized for Indian Healthcare.

Every kit component is engineered to withstand tropical climates and provides laboratory-grade results without the laboratory.

Core Capabilities

Detection Time
NMT 1 min
Storage Temp
2-30°C
Sensitivity
100% (Gold Standard)
Incitation
30-60 mins post-bite
Efficacy & Scale

Extensively verified across India. Clinical validation completed successfully.

Inside The Box (50 Tests)

01Test Device
01Dropper
01Test Diluent
01Alcohol Swab
01Lancet
01Instructions

Secondary Verification

Confirm bite type at DHC or hospital level

Initial Screening

Identify venomous/non-venomous bites at village level

Clinical Evaluation

Confirm absence of venom prior to patient discharge

Ongoing Monitoring

Track venom neutralization during ASV treatment

Testing Principles

The Mechanism behind the Diagnostic Accuracy.

Our kit is engineered to provide medical-grade precision in emergency conditions, using sophisticated antigen-antibody captures that ensure zero margin for error.

Active Chromatography

Refining rapid detection through a proprietary 3-zone membrane protocol, optimized for Indian medical contexts.

Methodology

The kit uses immunochromatography with colloidal gold–labeled equine polyclonal antibodies for rapid detection.

Capture Mechanism

Sample venom forms an antigen–antibody complex, captured at the Test (T) line to form a visible red band.

Verification

A Control (C) line ensures proper performance. Result should be interpreted within a 2–20 minute window.

Detection Limit

Detects venom concentrations as low as 10 ng/mL, adhering to the highest global diagnostic benchmarks.

Scientific Evidence

Precision & Accuracy FAQs

Addressing the technical benchmarks that define our 100% sensitivity and specificity standards.

Sensitivity (100%) means the kit never misses a case where venom is present. By targeting broad protein families (the "antigen" as a whole), the kit ensures that even if the venom concentration is low or the species is slightly different, there is enough shared protein structure for the antibodies to "catch" it.

Technical Resources

Downloads & Documentation.

DLS Marketing & Distribution · Hyderabad, India

Partner With Us

Ready to bring this test kit to your facility?

Dharani Life Sciences is actively distributing the Snake Venom Rapid Test Kit across Indian states. Contact us for bulk orders or pilot requests.